Analysis Note

Positive ControlA549 cells

Application

Immunoblotting (2 g/ml, chemiluminescence)Immunofluorescence (1 g/ml)Immunoprecipitation (1 g/reaction)Paraffin Sections (2.5 g/ml, heat pre-treatment required)

General description

This Anti-MDM2 (Ab-3) Mouse mAb (4B11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-3).

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells. May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2.

Immunogen

Human

Epitope: within amino acids 383-491

full-length, recombinant human MDM2

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Marchetti, A., et al. 1995. J. Pathol.175, 31.Barak, Y., et al. 1993. EMBO. J.12, 461.Ladanyi, M., et al. 1993. Cancer Res.53, 16.Leach, F. S., et al. 1993. Cancer Res.53, 2231.Oliner, J. D., et al. 1993. Nature362, 857.Momand, J., et al. 1992. Cell69, 1237.Oliner, J. D., et al. 1992. Nature358, 80.Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.

May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)